| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nitric oxide synthase, brain;1.14.13.39;Constitutive NOS;NC-NOS;N |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human nNOS (neuronal)/NOS1 recombinant protein (Position: R19-E1320). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects nitric oxide synthase 1 (gene NOS1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 161.0 kDa, and the supplier reports an observed band at about 161 kDa.
Neuronal nitric oxide synthase (nNOS, NOS1) is a Ca2+/calmodulin-dependent enzyme that converts L-arginine to nitric oxide. In the brain and peripheral nerves, nitric oxide acts as an unconventional neurotransmitter, and nNOS is also expressed in skeletal muscle. It is studied in synaptic plasticity, neurotoxicity and neuromuscular physiology, and it can promote S-nitrosylation of target proteins.
| Target | Nitric oxide synthase 1 (gene NOS1; UniProt P29475, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 161.0 kDa |
| Observed MW | 161 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human nNOS (neuronal)/NOS1 fragment (Arg19–Glu1320), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nitric oxide synthase 1 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Zhang et al. (2021) SerpinA3N attenuates ischemic stroke injury by reducing apoptosis and neuroinflammation. CNS Neuroscience & Therapeutics. 10.1111/cns.13776
George Akash K. et al. (2018) Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling. Scientific Reports. 10.1038/s41598-018-23568-z
Saumya Singh et al. (2020) Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase. Assay and Drug Development Technologies. 10.1089/adt.2018.912
Yang Lu et al. (2018) Effects of Repetitive Transcranial Magnetic Stimulation on Astrocytes Proliferation and nNOS Expression in Neuropathic Pain Rats. Current Medical Science. 10.1007/s11596-018-1904-3
