| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD; P; transhydrogenase, mitochondrial; Nicotinamide nucleotide transhydrogenase; Pyridine nucleotide transhydrogenase; NNT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NNT recombinant protein (Position: C44-A350). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NAD(P) transhydrogenase, mitochondrial (gene NNT) in human, mouse, monkey, rat and pig samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 113.9 kDa, and the supplier reports an observed band at about 114 kDa.
Nicotinamide nucleotide transhydrogenase (NNT) is an inner mitochondrial membrane enzyme that uses the proton gradient to transfer reducing equivalents from NADH to NADP+, producing NADPH. This NADPH supports antioxidant systems such as glutathione and thioredoxin. NNT is studied in redox balance, adrenal function and metabolism, and a common C57BL/6J mouse strain lacks functional NNT.
| Target | NAD(P) transhydrogenase, mitochondrial (gene NNT; UniProt Q13423, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Monkey, Rat, Pig |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 113.9 kDa |
| Observed MW | 114 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Monkey, Rat, Pig) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, monkey kidney tissue, pig kidney tissue, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SiHa cells.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human NNT fragment (Cys44–Ala350), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse, monkey, rat and pig. UniProt places NAD(P) transhydrogenase, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Widely expressed with expression most readily detectable in adrenal, heart, kidney, thyroid and adipose tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Litao Nie et al. (2022) Vitamin C protects the spleen against PFOA-induced immunotoxicity. Science of the Total Environment. 10.1016/j.scitotenv.2022.161266
