| Field | Specification |
|---|---|
| Target | |
| Alternative names | Reticulon-4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5; RTN4; KIAA0886; NOGO |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Nogo A/RTN4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes reticulon-4 (gene RTN4) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 129.9 kDa, and the supplier reports an observed band at about 180 kDa.
Reticulon-4 (RTN4), also known as Nogo, is a membrane protein that shapes the tubular endoplasmic reticulum. Its long isoform, Nogo-A, is abundant in CNS myelin and inhibits axon growth through receptors such as NgR1, making it a major subject of research on limited axon regeneration after spinal cord and brain injury.
| Target | Reticulon-4 (gene RTN4; UniProt Q9NQC3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 129.9 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 4 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U-87MG cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human prostatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human prostatic cancer tissue.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Synthetic peptide from the N-terminal region of human nogo A/RTN4.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform A: is specifically expressed in brain and testis and weakly in heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yiping Huang et al. (2026) Nrf2 regulates angiogenesis in spinal cystic echinococcosis via RTN4. Microbiology Spectrum. 10.1128/spectrum.01028-25
Tao Liu et al. (2019) Icariside II affects hippocampal neuron axon regeneration and improves learning and memory in a chronic cerebral hypoperfusion rat model. International Journal of Clinical and Experimental Pathology. PMID: 31933890
Zhaozong Fu et al. (2013) Methylprednisolone inhibits Nogo-A protein expression after acute spinal cord injury. Neural Regeneration Research. 10.3969/j.issn.1673-5374.2013.05.003
Qing-Zhu Nie et al. (2010) Expression of Nogo-A on the retina in rat model with chronic ocular hypertension. International Journal of Ophthalmology. 10.3980/j.issn.2222-3959.2010.02.04
