| Field | Specification |
|---|---|
| Target | |
| Alternative names | NOL9; Polynucleotide 5'-hydroxyl-kinase NOL9; EC 2.7.1.-; Nucleolar protein 9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NOL9 recombinant protein (Position: E131-N636). Human NOL9 shares 64.8% amino acid (aa) sequence identity with mouse NOL9. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets polynucleotide 5'-hydroxyl-kinase NOL9 in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
NOL9 is a polynucleotide 5′-kinase that phosphorylates 5′-hydroxyl ends of RNA and DNA. In the nucleolus it is needed to process the 32S pre-rRNA, particularly within internal transcribed spacer 2, to produce mature 5.8S and 28S rRNAs. It is studied in ribosome biogenesis and 60S subunit maturation.
| Target | Polynucleotide 5'-hydroxyl-kinase NOL9 (gene NOL9; UniProt Q5SY16, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NOL9 fragment (Glu131–Asn636), expressed in E. coli. Sequence identity with the mouse ortholog: 64.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places polynucleotide 5'-hydroxyl-kinase NOL9 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
