| Field | Specification |
|---|---|
| Target | |
| Gene symbol | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2). |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone S4147RM), raised in Rabbit, recognizes NOS3. It is validated for Western Blot, Immunohistochemistry, ELISA.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, ELISA |
|---|---|
| WB | 1:1000-1:2000 |
| IHC-P | 1:200-1:2000 |
| ELISA | Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
See specifications table.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Clone | S4147RM |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen Region | 1035-1110 |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2). |
| Purification | Affinity purification |
| Concentration | Lot specific |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| UniProt # | P29474 |
| Gene ID | 4846 |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for NOS3 research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
