| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADPH oxidase 4;1.6.3.-;Kidney oxidase-1;KOX-1;Kidney superoxide-producing NADPH oxidase;Renal NAD (P)H-oxidase;NOX4;RENOX; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NOX4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone E-14 is a rabbit monoclonal antibody that recognizes NADPH oxidase 4 (gene NOX4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 66.9 kDa, and the supplier reports an observed band at about 67 kDa.
NADPH oxidase 4 (NOX4) is a constitutively active NOX isoform that produces mostly hydrogen peroxide rather than superoxide. It is found in many cell types, including kidney, vascular and fibroblast cells, and is strongly induced by TGF-β. NOX4 is studied in oxygen sensing, fibrosis, vascular signaling and cell differentiation.
| Target | NADPH oxidase 4 (gene NOX4; UniProt Q9NPH5, human) |
|---|---|
| Clone | E-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 66.9 kDa |
| Observed MW | 67 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with confirmed staining (IHC): rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human NOX4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NADPH oxidase 4 in the cytoplasm, endoplasmic reticulum membrane, cell membrane and cell junction. Tissue expression noted by UniProt (human): Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Feifei Feng et al. (2022) The Protective Role of Tanshinone IIA in Silicosis Rat Model via TGF-β1/Smad Signaling Suppression, NOX4 Inhibition and Nrf2/ARE Signaling Activation. Drug Design Development and Therapy. 10.2147/DDDT.S230572
Guo Jun et al. (2024) THRA1/PGC-1α/SIRT3 pathway regulates oxidative stress and is implicated in hypertension of maternal hypothyroid rat offspring. Hypertension Research. 10.1038/s41440-024-02023-9
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