| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phorbol-12-myristate-13-acetate-induced protein 1;PMA-induced protein 1;Immediate-early-response protein APR;Protein Noxa;PMAIP1;NOXA; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Noxa |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAFB-16 is a rabbit monoclonal antibody that recognizes phorbol-12-myristate-13-acetate-induced protein 1 (gene PMAIP1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 6.0 kDa, and the supplier reports an observed band at about 16 kDa.
NOXA (PMAIP1) is a BH3-only pro-apoptotic member of the BCL-2 family that selectively binds MCL1 and targets it for degradation. Induced by p53 and other stress signals, it helps release BAK and trigger mitochondrial apoptosis, and it is studied in cancer biology and drug-induced cell death.
| Target | Phorbol-12-myristate-13-acetate-induced protein 1 (gene PMAIP1; UniProt Q13794, human) |
|---|---|
| Clone | AAFB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 6.0 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human 293T cells, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human noxa.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phorbol-12-myristate-13-acetate-induced protein 1 in the mitochondrion. Tissue expression noted by UniProt (human): Highly expressed in adult T-cell leukemia cell line. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Yingtong et al. (2023) p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma. Experimental Hematology & Oncology. 10.1186/s40164-023-00381-7
Fan Pengyu et al. (2023) Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer. Frontiers in Oncology. 10.3389/fonc.2023.1178595
Wenhua Wang et al. (2025) Therapeutic Potential of Targeting PCLAF in Endometrial Cancer: Insights from Wnt/β-catenin and p53 Regulatory Mechanisms. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2025.152092
