| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear protein localization protein 4 homolog; Protein NPL4; NPLOC4; KIAA1499; NPL4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NPL4/NPLOC4 recombinant protein (Position: M1-N495). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nuclear protein localization protein 4 homolog (gene NPLOC4) in human, mouse and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 68.1 kDa, and the supplier reports an observed band at about 72 kDa.
NPLOC4 (also called NPL4) partners with UFD1 to form a cofactor of the ATPase VCP/p97. The VCP–UFD1–NPL4 complex binds ubiquitinated proteins and extracts them from membranes or complexes for proteasomal degradation, including in ER-associated degradation. It is also involved in spindle disassembly after mitosis and is studied in protein quality control.
| Target | Nuclear protein localization protein 4 homolog (gene NPLOC4; UniProt Q8TAT6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 68.1 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human Caco-2 cells, human PC-3 cells, human SH-SY5Y cells, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Immunogen
Recombinant human NPL4/NPLOC4 fragment (Met1–Asn495), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear protein localization protein 4 homolog in the cytoplasm, endoplasmic reticulum and nucleus. Tissue expression noted by UniProt (human): Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
