| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C; NPR3; ANPRC; C5orf23; NPRC |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Natriuretic Peptide Receptor C |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEDH-14 is a rabbit monoclonal antibody that recognizes atrial natriuretic peptide receptor 3 (gene NPR3) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 59.8 kDa, and the supplier reports an observed band at about 72 kDa.
Natriuretic peptide receptor 3 (NPR3, also called NPR-C) binds ANP, BNP and CNP with similar affinity but lacks guanylyl cyclase activity. It mainly acts as a clearance receptor that internalizes and removes natriuretic peptides, thereby controlling their local levels. It also influences bone growth and is studied in cardiovascular and skeletal biology.
| Target | Atrial natriuretic peptide receptor 3 (gene NPR3; UniProt P17342, human) |
|---|---|
| Clone | AEDH-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.8 kDa |
| Observed MW | 72 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, rat kidney tissue, rat heart tissue, mouse kidney tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human natriuretic Peptide Receptor C.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places atrial natriuretic peptide receptor 3 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Hu et al. (2016) Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis. Journal of Receptors and Signal Transduction. 10.3109/10799893.2016.1155068
