| Field | Specification |
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| Alternative Names | Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5 |
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| Conjugate | |
| Host | |
| Isotype | |
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| Reactivity | |
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| Target |
Overview
Anti-NPY5R Antibody is an antibody targeting Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5 (also reported as Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5).
- Immunogen/epitope region: 3rd intracellular loop.
- Homology note: Mouse, bovine, canis - identical; human - 15/16 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M).
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR025.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR025; Negative control: BLP-NR025.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
