| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear receptor subfamily 0 group B member 2; Orphan nuclear receptor SHP; Small heterodimer partner; Nr0b2; Shp |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Nr0b2 recombinant protein (Position: A54-R219). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nuclear receptor subfamily 0 group B member 2 (gene Nr0b2) in mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 28.8 kDa, and the supplier reports an observed band at about 28 kDa.
Small heterodimer partner (SHP, NR0B2) is an atypical nuclear receptor lacking a DNA-binding domain that binds and represses other nuclear receptors and transcription factors. In the liver it is induced by FXR and mediates feedback inhibition of bile acid synthesis genes such as Cyp7a1. It is studied in mouse models of bile acid, lipid and glucose metabolism.
| Target | Nuclear receptor subfamily 0 group B member 2 (gene Nr0b2; UniProt Q62227, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.8 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): RM-1 cells.
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant mouse Nr0b2 fragment (Ala54–Arg219), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places nuclear receptor subfamily 0 group B member 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Expressed in islets of Langerhans (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tao Jing et al. (2022) DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model. Frontiers in Endocrinology. 10.3389/fendo.2021.723623
Wenjing Mo et al. (2023) Noni (Morinda citrifolia L.) fruit polysaccharide ameliorated high-fat diet-induced obesity by modulating gut microbiota and improving bile acid metabolism. Journal of Functional Foods. 10.1016/j.jff.2023.105408
