| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear receptor subfamily 2 group E member 1; Nuclear receptor TLX; Protein tailless homolog; Tll; hTll; NR2E1; TLX |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NR2E1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24N10 is a rabbit monoclonal antibody that recognizes nuclear receptor subfamily 2 group E member 1 (gene NR2E1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 42.6 kDa, and the supplier reports an observed band at about 48 kDa.
TLX (NR2E1) is an orphan nuclear receptor that binds DNA as a monomer and mainly acts as a transcriptional repressor. It keeps neural stem cells in a self-renewing state in the developing and adult forebrain and is needed for normal retinal development. TLX is studied in neurogenesis and brain tumors.
| Target | Nuclear receptor subfamily 2 group E member 1 (gene NR2E1; UniProt Q9Y466, human) |
|---|---|
| Clone | 24N10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.6 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NR2E1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear receptor subfamily 2 group E member 1 in the nucleus. Tissue expression noted by UniProt (human): Brain specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
