| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear receptor subfamily 5 group A member 2;Alpha-1-fetoprotein transcription factor;B1-binding factor;hB1F;CYP7A promoter-binding factor;Hepatocytic transcription factor;Liver receptor homolog 1;LRH-1;NR5A2;B1F, CPF, FTF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NR5A2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABHO-14 is a rabbit monoclonal antibody that recognizes nuclear receptor subfamily 5 group A member 2 (gene NR5A2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 61.3 kDa, and the supplier reports an observed band at about 70 kDa.
LRH-1 (NR5A2) is a monomeric DNA-binding nuclear receptor that regulates genes of cholesterol and bile acid metabolism in the liver and intestine. It also supports early embryonic development and pluripotency and is studied in liver and pancreas biology, intestinal steroidogenesis and pancreatic cancer.
| Target | Nuclear receptor subfamily 5 group A member 2 (gene NR5A2; UniProt O00482, human) |
|---|---|
| Clone | ABHO-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.3 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, human A549 cells, human Caco-2 cells, human 293T cells, rat ovary tissue, rat liver tissue, mouse ovary tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NR5A2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear receptor subfamily 5 group A member 2 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
