| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natural resistance-associated macrophage protein 1; NRAMP 1; Solute carrier family 11 member 1; SLC11A1; LSH; NRAMP; NRAMP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects natural resistance-associated macrophage protein 1 (gene SLC11A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 59.9 kDa, and the supplier reports an observed band at about 120 kDa.
NRAMP1 (SLC11A1) is a proton-coupled divalent metal transporter located in the membrane of late endosomes and phagosomes in macrophages and neutrophils. By pumping metal ions such as Fe2+ and Mn2+ out of the phagosome, it starves intracellular pathogens like Salmonella, Leishmania and mycobacteria, and its gene is a classic host resistance locus.
| Target | Natural resistance-associated macrophage protein 1 (gene SLC11A1; UniProt P49279, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 59.9 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human 293T cells, human K562 cells, rat spleen tissue, mouse Hepa1/6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, mouse spleen tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Immunogen
Recombinant human NRAMP1/SLC11A1 fragment (Phe183–Ser549), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places natural resistance-associated macrophage protein 1 in the cytoplasmic vesicle, late endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hui Zhang et al. (2025) Peripheral Immune Cell Infiltration in the Hippocampus of Sepsis Mice. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.70872
