| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nebulin-related-anchoring protein; N-RAP; NRAP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NRAP recombinant protein (Position: M1-K1513). Human NRAP shares 86.8% amino acid (aa) sequence identity with mouse NRAP. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nebulin-related-anchoring protein (gene NRAP) in human, mouse and rat samples and is validated for Western blot and ELISA.
Nebulin-related anchoring protein (NRAP) is an actin-binding protein of striated muscle found at myotendinous junctions in skeletal muscle and intercalated discs in heart muscle. It is thought to anchor terminal actin filaments of myofibrils to the membrane and help transmit force, and it is studied in myofibril assembly and cardiomyopathy.
| Target | Nebulin-related-anchoring protein (gene NRAP; UniProt Q86VF7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NRAP fragment (Met1–Lys1513), expressed in E. coli. Sequence identity with the mouse ortholog: 86.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in cardiac and skeletal muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
