| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal; NRF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human NRF1 recombinant protein (Position: D246-Q503). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nuclear respiratory factor 1 (gene NRF1) in human, mouse and rat samples and is validated for ELISA, flow cytometry, IHC and Western blot. The predicted molecular weight is 53.5 kDa, and the supplier reports an observed band at about 65 kDa.
Nuclear respiratory factor 1 (NRF1) is a transcription factor that activates many nuclear genes needed for mitochondrial respiration and heme synthesis, as well as TFAM and other factors that copy and transcribe mitochondrial DNA. It works with PGC-1 coactivators in mitochondrial biogenesis and is studied in energy metabolism and cell growth. It is distinct from NFE2L1, also called NRF1.
| Target | Nuclear respiratory factor 1 (gene NRF1; UniProt Q16656, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 53.5 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human MDA-MB-231 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human ovary cancer tissue, mouse intestine tissue, mouse kidney tissue, mouse spleen tissue, rat heart tissue, rat kidney tissue, human appendicitis tissue, human gastric cancer tissue, human tonsil tissue, mouse cardiac muscle tissue, mouse liver tissue, rat brain tissue, rat intestine tissue, rat liver tissue, rat lung tissue, rat skeletal muscle tissue, rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human NRF1 fragment (Asp246–Gln503), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear respiratory factor 1 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed with strongest expression in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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