| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal; NRF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NRF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADGI-14 is a rabbit monoclonal antibody that recognizes nuclear respiratory factor 1 (gene NRF1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 53.5 kDa, and the supplier reports an observed band at about 116 kDa.
Nuclear respiratory factor 1 (NRF1) is a transcription factor that activates many nuclear genes needed for mitochondrial respiration and heme synthesis, as well as TFAM and other factors that copy and transcribe mitochondrial DNA. It works with PGC-1 coactivators in mitochondrial biogenesis and is studied in energy metabolism and cell growth. It is distinct from NFE2L1, also called NRF1.
| Target | Nuclear respiratory factor 1 (gene NRF1; UniProt Q16656, human) |
|---|---|
| Clone | ADGI-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.5 kDa |
| Observed MW | 116 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human MOLT-4 cells, human U251 cells, rat brain tissue, human PC-12 cells, mouse brain tissue, human NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human bladder cancer tissue, human breast cancer tissue, human clear cells renal cells carcinoma tissue, human colon cancer tissue, human glioma tissue, human lymphoma tissue, human non-small-cells lung cancer tissue, human squamous cells carcinoma tissue, human testis cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human NRF1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear respiratory factor 1 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed with strongest expression in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
