| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; Nrf-2; Nuclear factor, erythroid derived 2, like 2; NFE2L2; NRF2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets nuclear factor erythroid 2-related factor 2 (gene NFE2L2) in human samples and is validated for Western blot, flow cytometry and ELISA.
NRF2 (NFE2L2) is a CNC-bZIP transcription factor that controls the cellular antioxidant response. Under normal conditions KEAP1 targets it for degradation, but oxidative or electrophilic stress stabilizes NRF2, which then binds antioxidant response elements to induce detoxifying and cytoprotective genes, making it central to redox biology.
| Target | Nuclear factor erythroid 2-related factor 2 (gene NFE2L2; UniProt Q16236, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NRF2/NFE2L2 fragment (Glu67–Asp601), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nuclear factor erythroid 2-related factor 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Wei et al. (2026) Systematic toxicology reveals dioctyl terephthalate as a hazardous agent on autism spectrum disorder-like behaviors: Evidence from network toxicology and zebrafish models. Journal of Environmental Sciences. 10.1016/j.jes.2026.03.015
Shilin Zhang et al. (2026) Hydroxytyrosol Enhances the Nrf2/HO-1 Signalling Pathway to Inhibit Oxidative Stress and Apoptosis and Improve Premature Ovarian Insufficiency In Vitro and In Vivo. International Journal of Molecular Sciences. 10.3390/ijms27114845
Qin Qingqing et al. (2025) Sulfasalazine induces ferroptosis in osteosarcomas by regulating Nrf2/SLC7A11/GPX4 signaling axis. Scientific Reports. 10.1038/s41598-025-13324-5
Zhang Xin et al. (2024) Daphnetin protects neurons in an Alzheimer disease mouse model and normal rat neurons by inhibiting BACE1 activity and activating the Nrf2/HO-1 pathway. Journal of Neuropathology and Experimental Neurology. 10.1093/jnen/nlae043
