| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; Protein H105e3; NSDHL; H105E3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NSDHL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30N66 is a rabbit monoclonal antibody that recognizes sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (gene NSDHL) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 41.9 kDa, and the supplier reports an observed band at about 38 kDa.
NSDHL is a sterol-4α-carboxylate 3-dehydrogenase that removes C4 methyl groups during the post-squalene steps of cholesterol synthesis. It is located on the endoplasmic reticulum and lipid droplets. NSDHL mutations cause CHILD syndrome and CK syndrome, and it is studied in cholesterol biosynthesis.
| Target | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (gene NSDHL; UniProt Q15738, human) |
|---|---|
| Clone | 30N66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.9 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human A431 cells, human MCF-7 cells, human RT4 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NSDHL.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Brain, heart, liver, lung, kidney, skin and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
