| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NSE |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FBI-5 is a rabbit monoclonal antibody that recognizes gamma-enolase (gene ENO2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 47.3 kDa, and the supplier reports an observed band at about 47 kDa.
Gamma-enolase (ENO2), also known as neuron-specific enolase (NSE), is the enolase isoform found mainly in neurons and neuroendocrine cells. Besides its glycolytic role, it has reported neurotrophic effects, and NSE is widely used as a marker of neuronal and neuroendocrine cells in tissue studies.
| Target | Gamma-enolase (gene ENO2; UniProt P09104, human) |
|---|---|
| Clone | FBI-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.3 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human uterus cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide based on the sequence of human NSE.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places gamma-enolase in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sijun Li et al. (2023) Drug–drug interactions between propofol and ART drugs: Inhibiting neuronal activity by affecting glucose metabolism. CNS Neuroscience & Therapeutics. 10.1111/cns.14437
Lan Yi et al. (2023) Cotransplantation of NSCs and ethyl stearate promotes synaptic plasticity in PD rats by Drd1/ERK/AP-1 signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2023.117292
Qingdong Zhu et al. (2024) Lactate dehydrogenase-1 may play a key role in the brain energy disturbance caused by cryptococcal meningitis. Journal of Microbiology Immunology and Infection. 10.1016/j.jmii.2024.08.009
Dingwen Xu et al. (2022) Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork. Experimental Eye Research. 10.1016/j.exer.2022.109235
Shao Jian-Lin et al. (2010) H2S Protects Hippocampal Neurons from Anoxia–Reoxygenation Through cAMP-Mediated PI3K/Akt/p70S6K Cell-Survival Signaling Pathways. Journal of Molecular Neuroscience. 10.1007/s12031-010-9464-4
Jin Wenyang et al. (2020) Neuroprotective Effects of Grape Seed Procyanidins on Ethanol-Induced Injury and Oxidative Stress in Rat Hippocampal Neurons. Alcohol and Alcoholism. 10.1093/alcalc/agaa031
Yang Hu et al. (2015) Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro. Molecular Medicine Reports. 10.3892/mmr.2015.4553
