| Field | Specification |
|---|---|
| Target | |
| Alternative names | NSMAF; FAN; Protein FAN; Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NSMAF recombinant protein (Position: K51-L868). Human NSMAF shares 92.3% amino acid (aa) sequence identity with mouse NSMAF. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein FAN (gene NSMAF) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
NSMAF (also called FAN, factor associated with neutral sphingomyelinase activation) is a WD-repeat adaptor protein that binds the cytoplasmic region of TNF receptor 1. It couples TNF signaling to activation of neutral sphingomyelinase and ceramide production, and it is studied in TNF-induced signaling and inflammation.
| Target | Protein FAN (gene NSMAF; UniProt Q92636, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NSMAF fragment (Lys51–Leu868), expressed in E. coli. Sequence identity with the mouse ortholog: 92.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Ubiquitous.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
