| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 SUMO-protein ligase NSE2; E3 SUMO-protein transferase NSE2; MMS21 homolog; hMMS21; Non-structural maintenance of chromosomes element 2 homolog; Non-SMC element 2 homolog; NSMCE2; C8orf36; MMS21 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NSMCE2 recombinant protein (Position: F14-D228). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes E3 SUMO-protein ligase NSE2 (gene NSMCE2) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 27.9 kDa, and the supplier reports an observed band at about 34 kDa.
NSMCE2 (also called MMS21) is the SUMO E3 ligase subunit of the SMC5–SMC6 complex, which maintains genome stability. It sumoylates proteins involved in DNA repair and helps homologous recombination at DNA double-strand breaks and telomere maintenance in ALT cancer cells. It is studied in DNA damage response and chromosome biology.
| Target | E3 SUMO-protein ligase NSE2 (gene NSMCE2; UniProt Q96MF7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.9 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human HEL cells, human U2OS cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): NSMCE2 antibody was positively detected by FC in:.
Immunogen
Recombinant human NSMCE2 fragment (Phe14–Asp228), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 SUMO-protein ligase NSE2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
