| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5-cytosine rRNA methyltransferase NSUN4; 5-cytosine tRNA methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; mRNA cytosine C(5)-methyltransferase NSUN4; NSUN4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NSUN4 recombinant protein (Position: M1-K379). Human NSUN4 shares 86.3% amino acid (aa) sequence identity with mouse NSUN4. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 5-cytosine rRNA methyltransferase NSUN4 in human samples and is validated for Western blot and ELISA.
NSUN4 is a mitochondrial RNA methyltransferase that forms 5-methylcytosine in mitochondrial 12S rRNA and other RNAs. Together with MTERF4 it also helps assemble the mitochondrial ribosome, and it is studied in mitochondrial translation and oxidative phosphorylation.
| Target | 5-cytosine rRNA methyltransferase NSUN4 (gene NSUN4; UniProt Q96CB9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NSUN4 fragment (Met1–Lys379), expressed in E. coli. Sequence identity with the mouse ortholog: 86.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5-cytosine rRNA methyltransferase NSUN4 in the mitochondrion.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
