| Field | Specification |
|---|---|
| Target | |
| Alternative names | NSUN6; NOPD1; tRNA; cytosine(72-C(5-methyltransferase NSUN6; EC 2.1.1.-; NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family member 6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NSUN6 recombinant protein (Position: E40-E400). Human NSUN6 shares 82.3% amino acid (aa) sequence identity with mouse NSUN6. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tRNA (gene NSUN6) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
NSUN6 is a cytoplasmic RNA methyltransferase that adds 5-methylcytosine at position 72 in the acceptor stem of selected tRNAs, such as tRNA-Thr and tRNA-Cys. It recognizes the tRNA three-dimensional shape including the CCA end and has also been reported to methylate mRNAs. It is studied in tRNA modification.
| Target | TRNA (gene NSUN6; UniProt Q8TEA1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human NSUN6 fragment (Glu40–Glu400), expressed in E. coli. Sequence identity with the mouse ortholog: 82.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tRNA in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
