| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurotrophin-4; NT-4; Neurotrophin-5; NT-5; Neutrophic factor 4; NTF4; NTF5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NT-4/NTF4, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat NT-4/NTF4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes neurotrophin-4 (gene NTF4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 22.4 kDa, and the supplier reports an observed band at about 24 kDa.
Neurotrophin-4 (NT-4, NTF4) is a secreted neurotrophin that signals mainly through the TrkB receptor, which it shares with BDNF. It acts as a survival factor for peripheral sensory and sympathetic neurons and is studied in neural development, neuron maintenance and synaptic function.
| Target | Neurotrophin-4 (gene NTF4; UniProt P34130, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 22.4 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HEK293 cells, human SW620 cells, human HeLa cells, rat RH-35 cells, mouse spleen tissue, mouse thymus tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human NT-4/NTF4. Sequence identity with the mouse and rat orthologs: 83.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes neurotrophin-4 as a secreted protein. Tissue expression noted by UniProt (human): Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
