| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'; 3'; -deoxyribonucleotidase, mitochondrial; 5',3'-nucleotidase, mitochondrial; Deoxy-5'-nucleotidase 2; dNT-2; NT5M; DNT2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NT5M recombinant protein (Position: F53-D164). Human NT5M shares 93.8% amino acid (aa) sequence identity with mouse NT5M. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 5'(3')-deoxyribonucleotidase, mitochondrial (gene NT5M) in human, mouse and rat samples and is validated for Western blot and ELISA.
NT5M is a mitochondrial 5′(3′)-deoxyribonucleotidase that removes phosphate groups mainly from uracil and thymine nucleotide monophosphates. By trimming these nucleotides inside mitochondria, it is thought to help balance the dTTP pool used for mitochondrial DNA synthesis.
| Target | 5'(3')-deoxyribonucleotidase, mitochondrial (gene NT5M; UniProt Q9NPB1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NT5M fragment (Phe53–Asp164), expressed in E. coli. Sequence identity with the mouse ortholog: 93.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 5'(3')-deoxyribonucleotidase, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Highly expressed in heart, brain and skeletal muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
