| Field | Specification |
|---|---|
| Target | |
| Alternative names | Linker for activation of T-cells family member 2; Linker for activation of B-cells; Membrane-associated adapter molecule; Non-T-cell activation linker; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; LAT2; LAB; NTAL; WBS15; WBSCR15; WBSCR5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NTAL/LAT2 recombinant protein (Position: R46-S217). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets linker for activation of T-cells family member 2 (gene LAT2) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 26.6 kDa, and the supplier reports an observed band at about 27 kDa.
LAT2 (NTAL, LAB) is a transmembrane adaptor protein related to LAT that is expressed in mast cells, B cells, NK cells and myeloid cells. After immune receptor activation it becomes phosphorylated and recruits GRB2, helping to connect receptors such as the high-affinity IgE receptor to downstream signaling. It is studied in mast cell activation and B-cell signaling.
| Target | Linker for activation of T-cells family member 2 (gene LAT2; UniProt Q9GZY6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.6 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Raji cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human NTAL/LAT2 fragment (Arg46–Ser217), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places linker for activation of T-cells family member 2 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
