| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 4; MRC OX40; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; Ox40; Txgp1l |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NTAL/LAT2 recombinant protein (Position: R46-S217). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NTAL/LAT2 Antibody Picoband® is an antibody for LAT2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: LAT2 (TNF receptor superfamily member 4); UniProt: Q9GZY6
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 27 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NTAL/LAT2 Antibody Picoband® catalog # A02496-1.
Biological background
Biological context: Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity). .
Expression and localization notes: cellular localization: Membrane; Single-pass type I membrane protein..
Common research applications
- Western blotting (WB): Compare LAT2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of LAT2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify LAT2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Linker for activation of T-cells family member 2, also known as WBSCR5 or NTAL is a protein that in humans is encoded by the LAT2 gene. It is mapped to 7q11.23. It has been found that NTAL associated with GRB2, SOS1 and GAB1 after B-cell receptor activation. Phosphorylation of NTAL was strongest in the presence of LCK, ZAP70, SYK, or LYNLAT2. NTAL is a structural and possibly a functional homolog of LAT in non-T cells. It also has been found that BLNK and LAT2 in B cells are a functionally equivalent pair to SLP76 and LAT in T cells. This gene consists of at least 14 exons, and its alternative splicing generates 3 transcript variants, all encoding the same protein.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane; Single-pass type I membrane protein.
- Research category: Adaptive Immunity,Autoimmune,B Cells,Cytokines,Hematopoietic Progenitors,Immune System Diseases,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
