| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleotide triphosphate diphosphatase NUDT15; MutT homolog 2; MTH2; Nucleoside diphosphate-linked moiety X motif 15; Nudix motif 15; Nucleoside diphosphate-linked to another moiety X hydrolase 15; Nudix hydrolase 15; NUDT15 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NUDT15 recombinant protein (Position: C23-L164). Human NUDT15 shares 90.8% amino acid (aa) sequence identity with mouse NUDT15. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects nucleotide triphosphate diphosphatase NUDT15 in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 18.6 kDa, and the supplier reports an observed band at about 19 kDa.
NUDT15 is a Nudix hydrolase that breaks down nucleoside triphosphates, including the active thiopurine metabolites 6-thio-GTP and 6-thio-dGTP. By inactivating these metabolites it limits thiopurine toxicity, and NUDT15 gene variants are a well-known factor in thiopurine drug tolerance.
| Target | Nucleotide triphosphate diphosphatase NUDT15 (gene NUDT15; UniProt Q9NV35, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 18.6 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Recombinant human NUDT15 fragment (Cys23–Leu164), expressed in E. coli. Sequence identity with the mouse ortholog: 90.8%.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
