| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear mitotic apparatus protein 1; Nuclear matrix protein-22; NMP-22; Nuclear mitotic apparatus protein; NuMA protein; SP-H antigen; NUMA1; NMP22; NUMA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NuMA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEBF-14 is a rabbit monoclonal antibody that recognizes nuclear mitotic apparatus protein 1 (gene NUMA1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 238.3 kDa, and the supplier reports an observed band at about 270 kDa.
NuMA (NUMA1) is a large microtubule-binding protein that is nuclear in interphase and moves to the spindle poles in mitosis. It tethers microtubule minus ends at the poles and works with dynein and LGN to orient the spindle, so it is studied in cell division, asymmetric division and nuclear organization.
| Target | Nuclear mitotic apparatus protein 1 (gene NUMA1; UniProt Q14980, human) |
|---|---|
| Clone | AEBF-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 238.3 kDa |
| Observed MW | 270 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human SH-SY5Y cells, human Hacat cells, human MCF-7 cells, human HEK293- WT cells, human HEK293-NUMA1 KO cells, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissueIHC:human bladder cancer tissue, human breast cancer tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human nuMA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear mitotic apparatus protein 1 in the nucleus, nucleus matrix, chromosome and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Biao Yu et al. (2019) Abnormality of intestinal cholesterol absorption in ApcMin/+ mice with colon cancer cachexia. International Journal of Clinical and Experimental Pathology. PMID: 31933883
