| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleoporin p54; 54 kDa nucleoporin; NUP54 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NUP54 recombinant protein (Position: D132-Q465). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets nucleoporin p54 (gene NUP54) in human samples and is validated for Western blot and ELISA.
Nup54 is a member of the central channel Nup62 subcomplex of the nuclear pore complex. Its phenylalanine-glycine (FG) repeats help form the permeability barrier through which transport receptors carry cargo between the nucleus and cytoplasm.
| Target | Nucleoporin p54 (gene NUP54; UniProt Q7Z3B4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NUP54 fragment (Asp132–Gln465), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nucleoporin p54 in the nucleus, nuclear membrane and nucleoplasmic side.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
