| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear pore glycoprotein p62; 62 kDa nucleoporin; Nucleoporin Nup62; NUP62 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NUP62 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26N26 is a rabbit monoclonal antibody that recognizes nuclear pore glycoprotein p62 (gene NUP62) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 53.3 kDa, and the supplier reports an observed band at about 70 kDa.
Nup62 is an O-GlcNAc-modified FG-repeat nucleoporin at the central channel of the nuclear pore complex, where it is central to nucleocytoplasmic transport. Outside the pore, it also associates with centrosomes and contributes to centrosome segregation and spindle orientation during mitosis.
| Target | Nuclear pore glycoprotein p62 (gene NUP62; UniProt P37198, human) |
|---|---|
| Clone | 26N26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.3 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:8000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human PC-3 cells, human HeLa cells, human 293T cells, human Jurkat cells, rat testis tissue, rat RH35 cells, mouse testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human NUP62.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nuclear pore glycoprotein p62 in the nucleus, cytoplasm and nucleus envelope. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
