| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear pore complex protein Nup98-Nup96; NUP98; ADAR2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NUP98 recombinant protein (Position: H728-I1787). Human NUP98 shares 89.1% and 89% amino acid (aa) sequence identity with mouse and rat NUP98, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects nuclear pore complex protein Nup98-Nup96 in human, monkey, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 197.6 kDa; the supplier reports observed bands at 98,105 kDa.
Nup98 is produced with Nup96 from a single precursor that is cleaved by self-processing; Nup98 is a mobile FG-repeat nucleoporin involved in two-way transport through the nuclear pore complex. It also influences gene expression inside the nucleus, and NUP98 fusion proteins are well-known drivers of acute myeloid leukemia.
| Target | Nuclear pore complex protein Nup98-Nup96 (gene NUP98; UniProt P52948, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 197.6 kDa |
| Observed MW | 98,105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, human HeLa cells, human K562 cells, monkey COS-7 cells, rat NRK cells, rat PC-12 cells, mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human NUP98 fragment (His728–Ile1787), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89.1% and 89%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places nuclear pore complex protein Nup98-Nup96 in the nuclear membrane, nucleoplasmic side and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
