| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3;NR4A1;GFRP1, HMR, NAK1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human NUR77 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NR4A1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABGF-14; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-NUR77 NR4A1 Rabbit Monoclonal Antibody catalog # M00626. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: NR4A1 (Nuclear receptor subfamily 4 group A member 1).
- Antibody format: Monoclonal; clone ABGF-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NR4A1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. . Reported cellular localization context: Cytoplasm. Nucleus. Tissue expression notes (as provided): Fetal muscle and adult liver, brain and thyroid.
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Cancer,Cell Biology,Cell Death,Co-Activators/Co-Repressors,Epigenetics and Nuclear Signaling,Invasion/Microenvironment,Nuclear Receptors,Nuclear Signaling Pathways,Nucleus.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate NR4A1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NR4A1 expression by Western blot in cell or tissue lysates, Compare relative NR4A1 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 26 kDa; calculated MW: 64463 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 26 kDa
- Cellular localization (provided): Cytoplasm. Nucleus.
- Tissue details (provided): Fetal muscle and adult liver, brain and thyroid.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
