| Field | Specification |
|---|---|
| Target | |
| Alternative names | 2'-5'-oligoadenylate synthase 3; (2-5')oligo (A) synthase 3; 2-5A synthase 3; p100 OAS; p100OAS; OAS3; P/OKcl.4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human OAS3 recombinant protein (Position: N990-V1087). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 2'-5'-oligoadenylate synthase 3 (gene OAS3) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 121.2 kDa, and the supplier reports an observed band at about 123 kDa.
OAS3 is an interferon-induced 2′-5′-oligoadenylate synthetase activated by double-stranded RNA. The 2′-5′-linked oligoadenylates it produces activate RNase L to degrade viral and cellular RNA, and OAS3 is considered the main OAS family member driving RNase L activation during many viral infections.
| Target | 2'-5'-oligoadenylate synthase 3 (gene OAS3; UniProt Q9Y6K5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 121.2 kDa |
| Observed MW | 123 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human HepG2 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human OAS3 fragment (Asn990–Val1087), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 2'-5'-oligoadenylate synthase 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Present at high level in placenta trophoblast. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-Zhou Huang et al. (2022) OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augmenting IFN-1‒Induced Jak1‒Signal Transducer and Activator of Transcription 1 Phosphorylation in Psoriasis. Journal of Investigative Dermatology. 10.1016/j.jid.2022.02.018
Lifang Wu et al. (2016) Preparation and characterization of latex films photo-immobilized with IFN-α. Colloids and Surfaces B-Biointerfaces. 10.1016/j.colsurfb.2016.04.037
