| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-OCTN1/SLC22A4 Antibody is an antibody targeting Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4 (also reported as Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4).
- Immunogen/epitope region: Intracellular, C-terminus..
- Homology note: Mouse, rat - 14 out of 15 amino acid residues identical; Human - 12 out of 15 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Cited use: IHC (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Organic Cation Transporter Novel (OCTN) is a subfamily of the larger Organic Cation Transporter (OCT) or SLC22 transporter family.The OCTN subfamily consists of two members in humans: OCTN1 (SLC22A4) and OCTN2 (SLC22A5) with a third member (SLC22A21) present only in rodents 1-2.The physiological role of OCTN1 (SLC22A4) has not yet been elucidated. OCTN1 is ubiquitously expressed throughout the body and transports various therapeutic agents, including organic cations and zwitterions. Ergothioneine (ET/ ERGO) a natural diet-derived antioxidant was found to be a specific substrate of OCTN1 3.OCTN1 is functionally expressed in neurons and neural stem cells and activation of the transporter could conceivably have a protective role in neurodegenerative diseases.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-CT014.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CT014; Negative control: BLP-CT014.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
