| Field | Specification |
|---|---|
| Target | |
| Alternative names | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human OGT / O-Linked N-Acetylglucosamine Transferase Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEDG-15 is a rabbit monoclonal antibody that recognizes UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (gene OGT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 116.9 kDa, and the supplier reports an observed band at about 110 kDa.
O-GlcNAc transferase (OGT) adds single N-acetylglucosamine sugars (O-GlcNAc) to serine and threonine residues of thousands of nuclear and cytoplasmic proteins. Using UDP-GlcNAc as a nutrient-sensitive donor, it regulates transcription, signaling and metabolism, and it is studied in diabetes, cancer and neurodegeneration.
| Target | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (gene OGT; UniProt O15294, human) |
|---|---|
| Clone | AEDG-15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.9 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human HeLa cells, human 293T cells, rat thymus tissue, rat spleen tissue, mouse thymus tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human OGT / O-Linked N-Acetylglucosamine Transferase Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
