| Field | Specification |
|---|---|
| Target | |
| Alternative names | Olfactomedin-4; OLM4; Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD; OLFM4; GW112 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human OLFM4 An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFEB-15 is a rabbit monoclonal antibody that recognizes olfactomedin-4 (gene OLFM4) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 54 kDa.
Olfactomedin-4 (OLFM4) is a secreted glycoprotein of the olfactomedin family. It is a well-known marker of intestinal stem cells and is also expressed in neutrophils, and it is studied in cell adhesion, innate immunity, inflammation and cancer.
| Target | Olfactomedin-4 (gene OLFM4; UniProt Q6UX06, human) |
|---|---|
| Clone | AFEB-15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.3 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:40 |
Samples with a confirmed band (WB): LnCaP cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human OLFM4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes olfactomedin-4 as secreted and also located in the mitochondrion. Tissue expression noted by UniProt (human): Expressed during myeloid lineage development. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
