| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oligodendrocyte transcription factor 2; Oligo2; Class B basic helix-loop-helix protein 1; bHLHb1; Class E basic helix-loop-helix protein 19; bHLHe19; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; OLIG2; PRKCBP2; RACK17 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human OLIG2, which shares 95% amino acid (aa) sequence identity with mouse OLIG2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects oligodendrocyte transcription factor 2 (gene OLIG2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 32.4 kDa, and the supplier reports an observed band at about 40 kDa.
OLIG2 is a basic helix-loop-helix transcription factor required to specify both motor neurons and oligodendrocytes from the pMN domain of the developing spinal cord. It is a standard marker of the oligodendrocyte lineage and is widely used in studies of gliogenesis, myelination and diffuse gliomas.
| Target | Oligodendrocyte transcription factor 2 (gene OLIG2; UniProt Q13516, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 32.4 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human THP-1 cells, rat brain tissue, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human OLIG2. Sequence identity with the mouse ortholog: 95%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places oligodendrocyte transcription factor 2 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in the brain, in oligodendrocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
