| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oncostatin-M; OSM |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Oncostatin M |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25O10 is a rabbit monoclonal antibody that recognizes oncostatin-M (gene OSM) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 28.5 kDa, and the supplier reports an observed band at about 22 kDa.
Oncostatin M (OSM) is an IL-6 family cytokine produced mainly by activated T cells, monocytes and neutrophils. It signals through gp130 paired with OSMR or LIFR to activate JAK–STAT pathways, and it is studied in inflammation, liver regeneration, fibrosis, bone remodeling and hematopoiesis.
| Target | Oncostatin-M (gene OSM; UniProt P13725, human) |
|---|---|
| Clone | 25O10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.5 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human oncostatin M.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes oncostatin-M as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
