| Field | Specification |
|---|---|
| Target | |
| Alternative names | Optineurin; E3-14.7K-interacting protein; FIP-2; Huntingtin yeast partner L; Huntingtin-interacting protein 7;HIP-7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Transcription factor IIIA-interacting protein; TFIIIA-IntP; OPTN; FIP2; GLC1E; HIP7; HYPL; NRP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3D8 is a mouse monoclonal antibody that recognizes optineurin (gene OPTN) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 65.9 kDa, and the supplier reports an observed band at about 66 kDa.
Optineurin (OPTN) is an adaptor protein involved in Golgi maintenance, membrane trafficking and selective autophagy, including mitophagy. It binds ubiquitin and LC3, recruits TBK1 in antiviral signaling and regulates NF-κB, and OPTN mutations are linked to glaucoma and amyotrophic lateral sclerosis.
| Target | Optineurin (gene OPTN; UniProt Q96CV9, human) |
|---|---|
| Clone | 3D8 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 65.9 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human U-87MG cells, human U2OS cells, human placenta tissue, human HeLa cells, human A549 cells, human K562 cells, rat C6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Optineurin fragment (Arg241–Ile577), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places optineurin in the cytoplasm, Golgi apparatus, cytoplasmic vesicle and recycling endosome. Tissue expression noted by UniProt (human): Present in aqueous humor of the eye (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
