| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcium release-activated calcium channel protein 1; Protein orai-1; Transmembrane protein 142A; ORAI1; CRACM1; TMEM142A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Orai1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17O87 is a rabbit monoclonal antibody that recognizes calcium release-activated calcium channel protein 1 (gene ORAI1) in human samples and is validated for Western blot. The predicted molecular weight is 32.7 kDa, and the supplier reports an observed band at about 33 kDa.
ORAI1 is the pore-forming subunit of the calcium release-activated calcium (CRAC) channel in the plasma membrane. When ER calcium stores are depleted, the sensor STIM1 opens ORAI1 to allow store-operated calcium entry, which is essential for T-cell activation, and ORAI1 loss causes a severe combined immunodeficiency.
| Target | Calcium release-activated calcium channel protein 1 (gene ORAI1; UniProt Q96D31, human) |
|---|---|
| Clone | 17O87 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.7 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human platelet.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human orai1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places calcium release-activated calcium channel protein 1 in the cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Expressed in naive CD4 and CD8 T cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
