| Field | Specification |
|---|---|
| Target | |
| Alternative names | Origin recognition complex subunit 5; ORC5; ORC5L |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ORC5, identical to the related mouse sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets origin recognition complex subunit 5 (gene ORC5) in human, mouse, rat and monkey samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 50.3 kDa, and the supplier reports an observed band at about 50 kDa.
ORC5 is an ATP-binding subunit of the origin recognition complex, which binds replication origins and recruits the factors needed to license DNA replication. It is studied in DNA replication initiation and genome stability.
| Target | Origin recognition complex subunit 5 (gene ORC5; UniProt O43913, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 50.3 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): monkey COS-7 cells, human placenta tissue, human K562 cells, human MCF-7 cells, rat testis tissue, rat kidney tissue, mouse ovary tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Synthetic peptide from the middle region of human ORC5. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. UniProt places origin recognition complex subunit 5 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Abundant in spleen, ovary, prostate, testis, and colon mucosa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Róża K Przanowska et al. (2025) DNA replication in primary hepatocytes without the six-subunit ORC. eLife. 10.7554/eLife.102915
