| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxysterol-binding protein-related protein 1; ORP-1; OSBP-related protein 1; OSBPL1A; ORP1; OSBP8; OSBPL1; OSBPL1B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ORP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27O45 is a rabbit monoclonal antibody that recognizes oxysterol-binding protein-related protein 1 (gene OSBPL1A) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 108.5 kDa; the supplier reports observed bands at 50, 108 kDa.
ORP1 (OSBPL1A) is an oxysterol-binding protein-related protein that binds sterols and phospholipids. Its long form associates with RAB7 on late endosomes and lysosomes, where it influences endosome positioning and cholesterol transfer to the ER.
| Target | Oxysterol-binding protein-related protein 1 (gene OSBPL1A; UniProt Q9BXW6, human) |
|---|---|
| Clone | 27O45 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 108.5 kDa |
| Observed MW | 50, 108 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ORP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places oxysterol-binding protein-related protein 1 in the late endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
