| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia up-regulated protein 1; 150 kDa oxygen-regulated protein; ORP-150; 170 kDa glucose-regulated protein; GRP-170; Heat shock protein family H member 4; HYOU1; GRP170; HSPH4; ORP150 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ORP150 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19H99 is a rabbit monoclonal antibody that recognizes hypoxia up-regulated protein 1 (gene HYOU1) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 111.3 kDa, and the supplier reports an observed band at about 150 kDa.
Hypoxia up-regulated protein 1 (HYOU1, also called ORP150 or GRP170) is a chaperone of the HSP70 superfamily located in the endoplasmic reticulum. It acts as a nucleotide exchange factor for BiP/GRP78, supports protein folding under stress and is induced by oxygen deprivation, so it is studied in ER stress, the unfolded protein response and cell survival under hypoxia.
| Target | Hypoxia up-regulated protein 1 (gene HYOU1; UniProt Q9Y4L1, human) |
|---|---|
| Clone | 19H99 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 111.3 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human MCF-7 cells, human 293T cells, human SH-SY5Y cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ORP150.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxia up-regulated protein 1 in the endoplasmic reticulum lumen. Tissue expression noted by UniProt (human): Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
