| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxysterol-binding protein 1;OSBP;OSBP1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human OSBP1, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets oxysterol-binding protein 1 (gene OSBP) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 89.4 kDa, and the supplier reports an observed band at about 75 kDa.
Oxysterol-binding protein 1 (OSBP) is a lipid transfer protein that works at contact sites between the ER and the Golgi. It swaps cholesterol for PI4P, carrying sterol to the Golgi and returning PI4P to the ER for degradation, and it is studied in lipid homeostasis and as a host factor used by several RNA viruses.
| Target | Oxysterol-binding protein 1 (gene OSBP; UniProt P22059, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 89.4 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat kidney tissue, rat spleen tissue, rat lung tissue, HeLa cells, A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human OSBP1. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places oxysterol-binding protein 1 in the cytoplasm, Golgi apparatus membrane, endoplasmic reticulum membrane and Golgi apparatus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Anna M. Kolodziejek et al. (2019) Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity. Cell Reports. 10.1016/j.celrep.2019.04.021
