| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oncostatin-M-specific receptor subunit beta; Interleukin-31 receptor subunit beta; IL-31 receptor subunit beta; IL-31R subunit beta; IL-31R-beta; IL-31RB; Osmr; Osmrb |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Osmr recombinant protein (Position: E673-Q968). Mouse Osmr shares 57.7% and 86.9% amino acid (aa) sequence identity with human and rat Osmr, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes oncostatin-M-specific receptor subunit beta (gene Osmr) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 110.2 kDa; the supplier reports observed bands at 110,180 kDa.
OSMR (oncostatin M receptor β) is a cytokine receptor subunit shared by two complexes: with gp130 it forms the type II oncostatin M receptor, and with IL31RA it forms the IL-31 receptor. Through JAK–STAT signaling it regulates inflammation and tissue responses, and it is studied in itch, skin inflammation and fibrosis.
| Target | Oncostatin-M-specific receptor subunit beta (gene Osmr; UniProt O70458, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 110.2 kDa |
| Observed MW | 110,180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat C6 cells, rat L6 cells, mouse Neuro-2a cells, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): C2C12 cells.
Immunogen
Recombinant mouse Osmr fragment (Glu673–Gln968), expressed in E. coli. Sequence identity with the human and rat orthologs: 57.7% and 86.9%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places oncostatin-M-specific receptor subunit beta in the membrane. Tissue expression noted by UniProt (mouse): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
