| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteocalcin;Bone Gla protein;BGP;Gamma-carboxyglutamic acid-containing protein;Bglap; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Osteocalcin recombinant protein (Position: Y50-I95). Mouse Osteocalcin shares 69% and 68.9% amino acid (aa) sequence identity with human and rat Osteocalcin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects osteocalcin (gene Bglap) in mouse samples and is validated for IHC. The predicted molecular weight is 10.5 kDa.
Osteocalcin (BGLAP) is a small bone protein produced by osteoblasts that becomes γ-carboxylated in a vitamin K-dependent manner, allowing it to bind calcium and hydroxyapatite in bone matrix. It is one of the most abundant noncollagenous proteins of bone, and circulating osteocalcin is widely used as a marker of bone formation.
| Target | Osteocalcin (gene Bglap; UniProt P86546, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.5 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
|---|
Samples with confirmed staining (IHC): mouse tibia tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Osteocalcin fragment (Tyr50–Ile95), expressed in E. coli. Sequence identity with the human and rat orthologs: 69% and 68.9%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes osteocalcin as a secreted protein. Tissue expression noted by UniProt (mouse): Bone.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Hengjie Su et al. (2024) Porous Nano-Fiber Structure of Modified Electrospun Chitosan GBR Membranes Improve Osteoblast Calcium Phosphate Deposition in Osteoblast-Fibroblast Co-Cultures. Marine Drugs. 10.3390/md22040160
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