| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteocalcin;Bone Gla protein;BGP;Gamma-carboxyglutamic acid-containing protein;Bglap;Bglap2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes osteocalcin (gene Bglap) in rat samples and is validated for flow cytometry and IHC. The predicted molecular weight is 10.9 kDa.
Osteocalcin (BGLAP) is a small bone protein produced by osteoblasts that becomes γ-carboxylated in a vitamin K-dependent manner, allowing it to bind calcium and hydroxyapatite in bone matrix. It is one of the most abundant noncollagenous proteins of bone, and circulating osteocalcin is widely used as a marker of bone formation.
| Target | Osteocalcin (gene Bglap; UniProt P04640, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 10.9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Rat) |
Samples with confirmed staining (IHC): rat tibia tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): L6 cells.
Immunogen
Recombinant rat Osteocalcin fragment (Tyr50–Val99), expressed in E. coli. Sequence identity with the human and mouse orthologs: 80.9% and 68.9%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes osteocalcin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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