| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; BGLAP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Osteocalcin |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 32B73 is a rabbit monoclonal antibody that recognizes osteocalcin (gene BGLAP) in human samples and is validated for Western blot. The predicted molecular weight is 11.0 kDa, and the supplier reports an observed band at about 16 kDa.
Osteocalcin (BGLAP) is a small bone protein produced by osteoblasts that becomes γ-carboxylated in a vitamin K-dependent manner, allowing it to bind calcium and hydroxyapatite in bone matrix. It is one of the most abundant noncollagenous proteins of bone, and circulating osteocalcin is widely used as a marker of bone formation.
| Target | Osteocalcin (gene BGLAP; UniProt P02818, human) |
|---|---|
| Clone | 32B73 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.0 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human osteocalcin.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes osteocalcin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Bai et al. (2025) Cryogenic 3D printing of Heterogeneous Scaffolds with Capability to Spatially Tune Cellular Morphology of Mesenchymal Stem Cells for Integrated Osteochondral Regeneration. Materials Today Bio. 10.1016/j.mtbio.2025.102560
Nan Wang et al. (2025) Immunomodulatory mesoporous bioactive glass/interleukin-4 nanocomplex promotes bone regeneration. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.149869
Jianan Kang et al. (2026) miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2026.110844
He Qingjian et al. (2026) Alpha-2-macroglobulin attenuates glucocorticoid-induced osteonecrosis of the femoral head by enhancing osteogenesis and antioxidant defense via the Akt/Nrf2 signaling pathway. Journal of Molecular Histology. 10.1007/s10735-026-10861-9
