| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin;SPP1;BNSP, OPN;PSEC0156; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Osteopontin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CEO-19 is a rabbit monoclonal antibody that recognizes osteopontin (gene SPP1) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 35.4 kDa, and the supplier reports an observed band at about 35 kDa.
Osteopontin (SPP1) is a secreted, phosphorylated glycoprotein first identified as a major non-collagenous protein of bone, where it binds hydroxyapatite and mediates cell-matrix interactions. It also acts as a cytokine that promotes interferon-γ and IL-12 production, and it is widely studied in bone remodeling, inflammation, fibrosis and tumor progression.
| Target | Osteopontin (gene SPP1; UniProt P10451, human) |
|---|---|
| Clone | CEO-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.4 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human MCF-7 cells, mouse kidney tissue, mouse small intestine, mouse NIH/3T3 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): Vascular Smooth Muscle cells.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human osteopontin.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes osteopontin as a secreted protein. Tissue expression noted by UniProt (human): Detected in cerebrospinal fluid and urine (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Yang Lu et al. (2025) Targeting astrocytic CLC2(CLCN2) restores myelin regeneration through inhibition of SPP1/CD44 signaling pathway in leukoencephalopathy. Molecular Psychiatry. 10.1038/s41380-025-03159-x
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Jing-Hong Liu et al. (2023) Antioxidant Activities and Cytotoxicity of the Regulated Calcium Oxalate Crystals on HK-2 Cells of Polysaccharides from Gracilaria lemaneiformis with Different Molecular Weights. Foods. 10.3390/foods12051031
Francesca Lombardi et al. (2024) Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures. Cells. 10.3390/cells13030258
Meng Sun et al. (2024) Extracellular vesicles derived from dental follicle stem cells regulate tooth eruption by inhibiting osteoclast differentiation. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2024.1503481
